THE VITAMIN ACTIVITIES OF “PYRIDOXAL” AND “PYRIDOXAMINE”
نویسندگان
چکیده
منابع مشابه
THE VITAMIN Ba GROUP VIII. BIOLOGICAL ASSAY OF PYRIDOXAL, PYRIDOXAMINE, AND PYRIDOXINE*
Though various chemical and microbiological methods have been developed for the estimation of vitamin Bs in whole (2-4) or in part (5), animal assays are still necessary for testing the accuracy of these new methods as they apply to animal nutrition. It is also important that the relative potency of pyridoxine, pyridoxamine, and pyridoxal for animals be known. Two different purified rations hav...
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acetone), than found in earlier investigations. Mayer (1930) used a concentration of 66% for his determinations, but this is definitely inhibitory, though lese so for insoluble preparations than for soluble. The enzyme is remarkably stable before it becomes soluble and the activity of soluble preparations is retained well in the cold. The optimum temperature for enzyme action is much lower than...
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The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile...
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on pyridoxine or pyridoxamine as a sole source of carbon and nitrogen (1, 2). This transaminase contains no pyridoxal phosphate, and hence provides a simpler system for mechanistic studies than do the more complex, pyridoxal phosphate-containing transaminases. The enzyme binds pyridoxamine and pyridoxal with almost equal avidity; the latter is bound in part by an azomethine Iinkage to the e-ami...
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two groups of students were assigned as experimental and control ones, and were given instruction on directed reading-thinking activities and after some treatment, they were post-tested. although the initial pre-test did not show any significant differences, the final post-test result revealed that the cooperative reading comprehension helped the experimental group. the cooperative students’...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1944
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71972-8